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peroxide-sensitive fluorescent 367 probe c11-bodipy 581/591  (Thermo Fisher)


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    Thermo Fisher peroxide-sensitive fluorescent 367 probe c11-bodipy 581/591
    Peroxide Sensitive Fluorescent 367 Probe C11 Bodipy 581/591, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxide+sensitive+fluorescence+probe/peroxide+sensitive+fluorescent+367+probe+c11+bodipy+581+591/10__5114_slash_aoms_slash_185257-62-18-25
    Average 90 stars, based on 1 article reviews
    peroxide-sensitive fluorescent 367 probe c11-bodipy 581/591 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Fluorescence:

    Article Title: ROCK Inhibitor Y-27632 Increases the Cloning Efficiency of Limbal Stem/Progenitor Cells by Improving Their Adherence and ROS-Scavenging Capacity
    Article Snippet: The nucleus was counterstained with DAPI, and the staining was viewed under an Eclipse TE2000U microscope (Nikon, Tokyo, Japan). .. For the detection of intracellular ROS accumulation, the 3T3 feeder cells were removed by transient 0.05% trypsin– EDTA treatment, followed by three washes with PBS.27,28 The colonies were then loaded with 10 mM peroxide-sensitive fluorescence probe 2,7-dichlorodihydrofluorescein diacetate– acetyl ester (H2-DCFDA; Molecular Probes, Eugene, OR) for 30 min at 37 C. The images were observed using Nikon confocal microscopy at 488/520 nm. .. The total cellular GSH content was measured using the GSH Assay Kit (Beyotime, Haimen, China) according to the manufacturer’s instructions.

    Article Title: Nicotinamide suppresses bevacizumab-induced epithelial-mesenchymal transition of ARPE-19 cells by attenuating oxidative stress
    Article Snippet: .. Cells were incubated with the peroxide-sensitive fluorescence probe, 2,7-dichlorodihydrofluorescein diacetate, acetyl-ester (10 μmol/L; Molecular Probes, Eugene, OR, USA) to detect the expression level of ROS and the H 2 O 2 sensor, pentafluorobenzenesulfonyl fluorescein (5 mmol/L; Santa Cruz Biotechnology, Dallas, TX, USA) to measure the expression level of H 2 O 2 for 30min at 37°C. .. The fluorescence intensities of these two probes were measured using a Nikon confocal laser-scanning microscope.

    Confocal Microscopy:

    Article Title: ROCK Inhibitor Y-27632 Increases the Cloning Efficiency of Limbal Stem/Progenitor Cells by Improving Their Adherence and ROS-Scavenging Capacity
    Article Snippet: The nucleus was counterstained with DAPI, and the staining was viewed under an Eclipse TE2000U microscope (Nikon, Tokyo, Japan). .. For the detection of intracellular ROS accumulation, the 3T3 feeder cells were removed by transient 0.05% trypsin– EDTA treatment, followed by three washes with PBS.27,28 The colonies were then loaded with 10 mM peroxide-sensitive fluorescence probe 2,7-dichlorodihydrofluorescein diacetate– acetyl ester (H2-DCFDA; Molecular Probes, Eugene, OR) for 30 min at 37 C. The images were observed using Nikon confocal microscopy at 488/520 nm. .. The total cellular GSH content was measured using the GSH Assay Kit (Beyotime, Haimen, China) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Nicotinamide suppresses bevacizumab-induced epithelial-mesenchymal transition of ARPE-19 cells by attenuating oxidative stress
    Article Snippet: .. Cells were incubated with the peroxide-sensitive fluorescence probe, 2,7-dichlorodihydrofluorescein diacetate, acetyl-ester (10 μmol/L; Molecular Probes, Eugene, OR, USA) to detect the expression level of ROS and the H 2 O 2 sensor, pentafluorobenzenesulfonyl fluorescein (5 mmol/L; Santa Cruz Biotechnology, Dallas, TX, USA) to measure the expression level of H 2 O 2 for 30min at 37°C. .. The fluorescence intensities of these two probes were measured using a Nikon confocal laser-scanning microscope.

    Expressing:

    Article Title: Nicotinamide suppresses bevacizumab-induced epithelial-mesenchymal transition of ARPE-19 cells by attenuating oxidative stress
    Article Snippet: .. Cells were incubated with the peroxide-sensitive fluorescence probe, 2,7-dichlorodihydrofluorescein diacetate, acetyl-ester (10 μmol/L; Molecular Probes, Eugene, OR, USA) to detect the expression level of ROS and the H 2 O 2 sensor, pentafluorobenzenesulfonyl fluorescein (5 mmol/L; Santa Cruz Biotechnology, Dallas, TX, USA) to measure the expression level of H 2 O 2 for 30min at 37°C. .. The fluorescence intensities of these two probes were measured using a Nikon confocal laser-scanning microscope.



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    Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    peroxide-sensitive fluorescent 367 probe c11-bodipy 581/591  (Thermo Fisher)
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    Thermo Fisher peroxide-sensitive fluorescent 367 probe c11-bodipy 581/591
    Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced <t>(red)</t> <t>C11-BODIPY</t> staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Peroxide Sensitive Fluorescent 367 Probe C11 Bodipy 581/591, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxide+sensitive+fluorescence+probe/peroxide+sensitive+fluorescent+367+probe+c11+bodipy+581+591/10__5114_slash_aoms_slash_185257-62-18-25
    Average 90 stars, based on 1 article reviews
    peroxide-sensitive fluorescent 367 probe c11-bodipy 581/591 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

    doi: 10.1016/j.mtbio.2026.102965

    Figure Lengend Snippet: Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Lipid peroxidation in ovarian tissues was assessed using the lipid peroxidation sensitive fluorescent probe C11-BODIPY (581/591) (MedChemExpress, USA), which shifts from red to green fluorescence upon oxidation.

    Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence

    UC-MSCs alleviated cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 8). (C) Measurement of the Fe 2+ concentration in ovarian tissues via a colorimetric assay ( n = 6). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) CD31 immunohistochemical staining was used to assess microvessel density in ovarian tissue. Scale bar, 50 μm. (F) Quantitative analysis of CD31-positive microvessel density ( n = 5). (G) RT-qPCR analysis of the expression of angiogenesis-related genes, including VEGF, ANG2, and IGF1 ( n = 5). (H) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (I) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. Student's t -test or one-way ANOVA with LSD post hoc tests was used. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

    doi: 10.1016/j.mtbio.2026.102965

    Figure Lengend Snippet: UC-MSCs alleviated cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 8). (C) Measurement of the Fe 2+ concentration in ovarian tissues via a colorimetric assay ( n = 6). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) CD31 immunohistochemical staining was used to assess microvessel density in ovarian tissue. Scale bar, 50 μm. (F) Quantitative analysis of CD31-positive microvessel density ( n = 5). (G) RT-qPCR analysis of the expression of angiogenesis-related genes, including VEGF, ANG2, and IGF1 ( n = 5). (H) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (I) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. Student's t -test or one-way ANOVA with LSD post hoc tests was used. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Lipid peroxidation in ovarian tissues was assessed using the lipid peroxidation sensitive fluorescent probe C11-BODIPY (581/591) (MedChemExpress, USA), which shifts from red to green fluorescence upon oxidation.

    Techniques: Immunohistochemical staining, Staining, Concentration Assay, Colorimetric Assay, Quantitative RT-PCR, Expressing, Fluorescence

    MSC-EVs repaired cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 5). (C) Quantification of Fe 2+ concentrations in ovarian tissue via a colorimetric assay ( n = 5). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (F) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

    doi: 10.1016/j.mtbio.2026.102965

    Figure Lengend Snippet: MSC-EVs repaired cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 5). (C) Quantification of Fe 2+ concentrations in ovarian tissue via a colorimetric assay ( n = 5). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (F) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Lipid peroxidation in ovarian tissues was assessed using the lipid peroxidation sensitive fluorescent probe C11-BODIPY (581/591) (MedChemExpress, USA), which shifts from red to green fluorescence upon oxidation.

    Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence